BIOL 335: Homework 3 (Spring 2018)


BIOL 335: Homework 3 (Spring 2018)

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Review questions:

  1. Briefly, that figure illustrates the cell fusion experiment between a human and mouse cell in which membrane proteins on each cell were fluorescently labeled. You can use a sketch/sketches to help you answer the question. (3points)
    1. How would the results be different if the experiment were conducted at 0°C?

 

  1. How would the results be different if the human cell plasma membrane contained more cholesterol than the mouse cell?

 

  1. How would the results be different if the mouse cell plasma membrane contained more saturated hydrocarbons than the human cell?

 

  1. Describe the events that occur in the ER and Golgi that lead to the asymmetrical distribution of phospholipids in the plasma membrane of eukaryotic cells. You can use a sketch to help you. (2 points)

 

  1. Imagine a transmembrane protein made of 7 alpha helices. This protein functions as a pore that allows Cl- ions to pass through the membrane. Each alpha helix has hydrophobic amino acids on one surface and hydrophilic amino acids on the opposite surface. Describe how these helices are likely arranged in the membrane. (1 point)

 

Reading focus questions for our next class:

  1. Passive transporters mediate the transfer of a solute down their concentration gradeints across a membrane.
    1. Sketch a passive transporter. Be sure to label the transporter, membrane, direction of transport, and concentration gradient (1point)

 

  1. Now add a pump to your diagram that moves the solute against its concentration gradient by relying on ATP hydrolysis. Explain the need for each of the steps in this addition to your diagram.(1 point)

 

  1. Name at least one similarity and one difference between the following.
    1. Symport and antiport (1 point)

 

  1. Membrane potential and electrochemical gradient (1 point)

 

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